Real-time PCR, also known as quantitative PCR (qPCR), is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets.
Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.
Real-time PCR applications
Gene expression (mRNA) analysis
Detect and quantifying target genes, even at very low expression levels.
MicroRNA (miRNA) analysis
Solutions for miRNA research. miRNAs are small, noncoding RNA molecules that direct post-transcriptional suppression of gene expression.
Genetic variation analysis
Genetic variation refers to the differences in the DNA sequence encoded within our genomes. Specific applications include:
- SNP genotyping
- Drug metabolism enzyme (DME) genotyping
- Copy number variation (CNV) analysis
Protein expression analysis
TaqMan Protein assays combine the protein-binding of antibodies with the quantitation of real-time PCR. They enable fast, easy identification of proteins from small samples in a homogeneous assay format.
Mutation detection
Real-time PCR is a widely used technology for mutation detection that provides sensitivity and specificity when paired with TaqMan Assays and is fast and easy to perform compared to other methods. Explore these solutions:
- castPCR technology for detecting somatic mutations for cancer research
- FTR gene mutation analysis
- Biomarker analysis
Verification of NGS and microarray results
Together, RNA-Seq, microarrays, and qPCR provide a comprehensive workflow for gene expression analysis, from discovery and testing to downstream verification.
Real-time PCR research areas
Virology research
Detect specific viruses and verify targets involved in the immunological response to viral infection, including SARS-CoV-2 infection.
Immunology research
Detect and quantitate the expression levels of common and novel immunology biomarkers identified in transcriptomic studies.
Cancer research
Discover and verify oncology biomarkers with gold-standard specificity and sensitivity.
Immuno-oncology research
Discover and verify genomic biomarkers of well-understood checkpoint inhibitors and other immune-related genes.
Please click the link below to learn more.
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Post time: Sep-01-2022